鸡G3BP1在新城疫病毒感染诱导应激颗粒形成过程中的作用

doi:10.11843/j.issn.0366-6964.2017.03.015

Abstract

Abstract:

To investigate the mechanism of chicken stress granule (SG) formation and the role of Ras GTPase- activating protein-binding protein 1 (G3BP1) in SG formation and virus infection, immunofluorescence assay was utilized to evaluate G3BP1 accumulation in Newcastle disease virus (NDV) infected HeLa cells. GFP-tagged chicken G3BP1 and its 4 domains were transfected into HeLa cells, respectively, before exposed to various stressors. Finally, G3BP1 was transfected into cells before NDV infection. Western blot and TCID₅₀ test were utilized to evaluate virus replication. The results showed that NDV infection induced endogenous G3BP1 accumulating as SGs. Transfection of exogenous chicken G3BP1 into either HeLa, DF-1 or CEF cells induced SGs in the presence or absence of environmental stress. Then the role of 4 domains of chicken G3BP1 in SG formation was evaluated. The results showed that RNA-binding domain was responsible for SG accumulation. In contrast, NTF2-like domain inhibit SG formation. Finally, it confirmed that G3BP1 overexpression enhanced NDV replication, indicating the pro-viral role of SG in NDV infection. These results demonstrated that chicken G3BP1 plays an important role in SG formation. Chicken SG promotes NDV replication.This study lay the foundation for the interactions between NDV and chicken cells.

CLC Number:

S852.659.5

ZHANG Pin, SUN Ying-jie, ZHENG Hang, MAO Xu-ming, QIU Xu-sheng, TAN Lei, MENG Chun-chun, SONG Cui-ping, LIAO Ying, DING Chan, MA Wei-ming. The Role of Chicken G3BP1 in the Formation of Stress Granules Induced by Newcastle Disease Virus Infection[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(3): 515-521.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2017.03.015

https://www.xmsyxb.com/EN/Y2017/V48/I3/515

References

［1］ANDERSON P, KEDERSHA N. RNA granules［J］. J Cell Biol, 2006, 172(6): 803-808.
［2］KEDERSHA N, CHO M R, LI W, et al. Dynamic shuttling of TIA-1 accompanies the recruitment of mRNA to mammalian stress granules［J］. J Cell Biol, 2000, 151(6): 1257-1268.
［3］ARIMOTO K, FUKUDA H, IMAJOH-OHMI S, et al. Formation of stress granules inhibits apoptosis by suppressing stress-responsive MAPK pathways［J］. Nat Cell Biol, 2008, 10(11): 1324-1332.
［4］BUCHAN J R, PARKER R. Eukaryotic stress granules: the ins and outs of translation［J］. Mol Cell, 2009, 36(6): 932-941.
［5］KENNEDY D, FRENCH J, GUITARD E, et al. Characterization of G3BPs: tissue specific expression, chromosomal localisation and rasGAP(120) binding studies［J］. J Cell Biochem, 2001, 84(1): 173-187.
［6］KIMBALL S R, HORETSKY R L, RON D, et al. Mammalian stress granules represent sites of accumulation of stalled translation initiation complexes［J］. Am J Physiol Cell Physiol, 2003, 284(2): C273-C284.
［7］BULLOCK T L, CLARKSON W D, KENT H M, et al. The 1.6 angstroms resolution crystal structure of nuclear transport factor 2 (NTF2)［J］. J Mol Biol, 1996, 260(3): 422-431.
［8］GALLOUZI I E, PARKER F, CHEBLI K, et al. A novel phosphorylation-dependent RNase activity of GAP-SH3 binding protein: a potential link between signal transduction and RNA stability［J］. Mol Cell Biol, 1998, 18(7): 3956-3965.
［9］KENT H M, CLARKSON W D, BULLOCK T L, et al. Crystallization and preliminary X-ray diffraction analysis of nuclear transport factor 2［J］. J Struct Biol, 1996, 116(2): 326-329.
［10］TOURRIÈRE H, GALLOUZI I E, CHEBLI K, et al. RasGAP-associated endoribonuclease G3Bp: selective RNA degradation and phosphorylation-dependent localization［J］. Mol Cell Biol, 2001, 21(22): 7747-7760.
［11］TOURRIÈRE H, CHEBLI K, ZEKRI L, et al. The RasGAP-associated endoribonuclease G3BP assembles stress granules［J］. J Cell Biol, 2003, 160(6): 823-831.
［12］MCINERNEY G M, KEDERSHA N L, KAUFMAN R J, et al. Importance of eIF2alpha phosphorylation and stress granule assembly in alphavirus translation regulation［J］. Mol Biol Cell, 2005, 16(8): 3753-3763.
［13］RUGGIERI A, DAZERT E, METZ P, et al. Dynamic oscillation of translation and stress granule formation mark the cellular response to virus infection［J］. Cell Host Microbe, 2012, 12(1): 71-85.
［14］ONOMOTO K, YONEYAMA M, FUNG G, et al. Antiviral innate immunity and stress granule responses［J］. Trends Immunol, 2014, 35(9): 420-428.
［15］NG C S, JOGI M, YOO J S, et al. Encephalomyocarditis virus disrupts stress granules, the critical platform for triggering antiviral innate immune responses［J］. J Virol, 2013, 87(17): 9511-9522.
［16］QIN Q, HASTINGS C, MILLER C L. Mammalian orthoreovirus particles induce and are recruited into stress granules at early times postinfection［J］. J Virol, 2009, 83(21): 11090-11101.
［17］GARAIGORTA U, HEIM M H, BOYD B, et al. Hepatitis C virus (HCV) induces formation of stress granules whose proteins regulate HCV RNA replication and virus assembly and egress［J］. J Virol, 2012, 86(20): 11043-11056.
［18］KATOH H, OKAMOTO T, FUKUHARA T, et al. Japanese encephalitis virus core protein inhibits stress granule formation through an interaction with Caprin-1 and facilitates viral propagation［J］. J Virol, 2013, 87(1): 489-502.
［19］SIMPSON-HOLLEY M, KEDERSHA N, DOWER K, et al. Formation of antiviral cytoplasmic granules during orthopoxvirus infection［J］. J Virol, 2011, 85(4): 1581-1593.
［20］ROZELLE D K, FILONE C M, KEDERSHA N, et al. Activation of stress response pathways promotes formation of antiviral granules and restricts virus replication［J］. Mol Cell Biol, 2014, 34(11): 2003-2016.
［21］LINDQUIST M E, LIFLAND A W, UTLEY T J, et al. Respiratory syncytial virus induces host RNA stress granules to facilitate viral replication［J］. J Virol, 2010, 84(23): 12274-12284.
［22］ZHANG S, SUN Y, CHEN H, et al. Activation of the PKR/eIF2alpha signaling cascade inhibits replication of Newcastle disease virus［J］. Virol J, 2014, 11: 62-68.

[1]	MENG Lingzhai, CHEN Chunli, YU Mengmeng, WANG Zhanxin, WANG Suyan, LIU Peng, HE Tana, GUO Ru, CHEN Yuntong, LIU Changjun, QI Xiaole, WU Zhiqiang, GAO Yulong. Pathogenicity of Avian Metapneumovirus Subtype B on Yellow Feather Broilers and Evaluation on Immune Effect of Inactivated Vaccine [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5154-5161.
[2]	JIANG Shengqiang, HU Jing, CHEN Hongying. Expression Analysis of CircRNAs in A549 Cells Infected with H1N1 Influenza A Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4724-4734.
[3]	ZHANG Ao, TAN Bin, LIU Kexin, LIU Jiali, ZHANG Shuqin. Genomic Characterization Analysis of a H1N1 Subtype Swine Influenza Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4866-4871.
[4]	HE Chenxiang, GAO Shandian, TIAN Zhancheng, DU Junzheng, WANG Jinming, GUAN Guiquan, YIN Hong. Development of a Differential Diagnostic ELISA Based on G_NS Protein to Distinguish BEFV Infected and Vaccinated Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4320-4326.
[5]	CHANG Yiming, TANG Cheng, YUE Hua. Molecular Epidemiological Investigation of Bovine Respiratory Syncytial Virus in Yaks [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2030-2041.
[6]	FANG Yuan, HOU Qiaodi, XIANG Chaohui, ZHAO Hongyi, QI Xuefeng. Regulatory Effects of IFITM3 on Proliferation of Peste des Petits Ruminants Virus (PPRV) in Goat Endometrial Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2200-2207.
[7]	LI Shun, LI Guizhen, YUAN Yaoxian, LI Kangjian, MA Chunquan, LI Shoujun, HUANG Liangzong, MA Jun. Effect of the Length of PA-X Gene on Replication and Pathogenicity of H3N2 Canine Influenza Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 272-280.
[8]	CUI Mingxian, WANG Xingbo, HUANG Yanming, BIAN Xiyi, FENG Mengke, YAN Yan, DONG Weiren, ZHOU Jiyong. Genetic Characterization and Evolution of Three Strains of H3N2 Avian Influenza Viruses [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4116-4122.
[9]	YU Mengmeng, BAO Yuanling, WANG Suyan, XIN Ziqi, LIU Peng, FENG Xiaoyan, MENG Lingzhai, GUO Ru, ZHANG Yanping, LIU Changjun, QI Xiaole, LI Junping, WANG Xiaomei, GAO Yulong. Isolation, Identification and Pathogenicity of Subtype B Avian Metapneumovirus [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3540-3549.
[10]	ZHOU Yong, LI Zhixin, LU Hongwei, SUN Yan, LI Tian, DU Fanshu, PU Juan. Surveillance and Outbreak Analysis of H5 and H7N9 Subtypes of Highly Pathogenic Avian Influenza in China [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3093-3106.
[11]	PAN Chunrong, DENG Ruixue, HU Linjie, ZHU Xueliang, SUN Yuefeng, WANG Guirong, MENG Xuelian. Effect of Histone Deacetylase Inhibitors on Peste Des Petits Ruminants Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2307-2316.
[12]	CHEN Zixuan, ZHANG Nan, HU Qun, QUAN Keji, QIN Tao, CHEN Sujuan, PENG Daxin, LIU Xiufan. Mutation Analysis of Antigen Sites 145 and 153 on Hemagglutinin of H9N2 Avian Influenza Virus in China [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1165-1172.
[13]	DUAN Zhiqiang, XIE Lingling, CHEN Jiaqi, TANG Hong, WANG Yanbi, ZHAO Caiqin, ZHAO Jiafu. Research Progress on the Interactions of Newcastle Disease Virus M Protein with Host Proteins [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 32-42.
[14]	YAN Yayao, GU Min, LIU Xiufan. Advance in the Influence of Amino Acid Variation in HA Protein on the Biological Properties of H7N9 Subtype Influenza Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2093-2106.
[15]	JIANG Mei, CHEN Wu, ZHAI Junqiong, BU Wandi, XIE Yilun, LIU Canbin, SHAN Fen, LUO Manlin. Cloning and Sequence Analysis of H and F Genes of Canine Distemper Virus Strain from Red Panda [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1670-1676.

The Role of Chicken G3BP1 in the Formation of Stress Granules Induced by Newcastle Disease Virus Infection

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments